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Invitrogen
RANBP2 Monoclonal Antibody (2E1)
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Application
FIGURE: 1 / 3
RANBP2 Antibody (MA1-847) in ICC/IF
Immunofluorescent analysis of Nup358 (green) in HeLa cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 1% Blocker BSA in PBS (Product # 37525), each for 15 minutes at room temperature. Cells were stained with a Nup358 monoclonal antibody (Product # MA1-847) at a concentration of 10 µg/mL in 1% Blocker BSA in PBS, followed by a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were... View More
Product Details
MA1-847
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2E1
Immunogen
Recombinant protein corresponding to 150 N-terminal residues of human Nup358.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2176789
Product Specific Information
MA1-847 detects Nup358/RanBP2 from human samples.
Target Information
RanBP2 interacts with the small GTP-binding protein Ran and forms a large multiprotein complex involved in many biological processes such as mRNA processing, nuclear transport, cell cycle control and postmitotic nuclear reassembly. The protein is identified by western blotting as an apparent molecular mass of 360 kD. It is involved in nucleation of microtubule and control of cell growth by interacting with many protein factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 358 kDa nucleoporin; acute necrotizing encephalopathy 1 (autosomal dominant); E3 SUMO-protein ligase RanBP2; Nuclear pore complex protein Nup358; nucleoporin 358; Nucleoporin Nup358; P270; Ran-binding protein 2; RanBP2; RBP2; transformation-related protein 2
Gene Aliases: ADANE; ANE1; IIAE3; NUP358; RANBP2; TRP1; TRP2
UniProt ID: (Human) P49792
Entrez Gene ID: (Human) 5903
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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