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Invitrogen
RANKL (soluble) Polyclonal Antibody, Biotin, PeproTech®
FIGURE: 1 / 3
RANKL (soluble) Antibody (500-P133GBT-1MG) in WB
Western Blot: To detect hsRANKL by Western Blot analysis RANKL (soluble) Polyclonal Antibody, Biotin (Product # 500-P133GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P133GBT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 20.0 kDa Recombinant Human sRANK Ligand
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929410
Product Specific Information
AA Sequence of recombinant protein: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sRANK Ligand by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sRANK Ligand (500-P133G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sRANK Ligand.
Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P133GBT-1MG will be provided as 2 x 500 µg
Target Information
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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