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Invitrogen

RAP1A Monoclonal Antibody (1D2-1C64)

1 Reference

View all (16) RAP1A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

Application

RAP1A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

RAP1A Antibody (MA1-013) in ICC/IF

Immunofluorescent analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody (Product # MA1-013) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conj... View More

RAP1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAP1A Antibody in Immunoprecipitation (IP)

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Product Details

MA1-013

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:10-1:200

Immunoprecipitation (IP)

2 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1D2-1C64

Immunogen

Recombinant full-length human Rap1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536666

Product Specific Information

Western blot analysis of MA1-013 detects an ~21kDa protein.

Target Information

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ArhGEF12 activates Rap1A and not RhoA in human dermal microvascular endothelial cells to reduce tumor necrosis factor-induced leak.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Khan A,Ni W,Baltazar T,Lopez-Giraldez F,Pober JS,Pierce RW

MA1-013 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to conclude that in TJ-forming HDMECs, ArhGEF12 selectively activates Rap1A to limit capillary barrier disruption in a mechanism independent of cAMP-mediated Epac1 activation.

Fri Apr 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: C21KG; G-22K; GTP-binding protein smg p21A; GTP-binding protein SMG-P21A; Ras-related protein Krev-1; Ras-related protein Rap-1A

Gene Aliases: AI848598; C21KG; G-22K; KREV-1; KREV1; RAP1; RAP1A; SMGP21

UniProt ID: (Human) P62834, (Mouse) P62835

Entrez Gene ID: (Human) 5906, (Mouse) 109905

Function(s)

GTPase activity protein binding GTP binding protein transporter activity Ras GTPase binding Rap guanyl-nucleotide exchange factor activity protein complex binding nucleotide binding small GTPase

Process(es)

activation of MAPKK activity signal transduction positive regulation of neuron projection development protein transport microvillus assembly Rap protein signal transduction negative regulation of collagen biosynthetic process cellular response to drug nerve growth factor signaling pathway positive regulation of GTPase activity positive regulation of protein kinase activity positive regulation of glucose import positive regulation of phagocytosis regulation of insulin secretion establishment of endothelial barrier positive regulation of ERK1 and ERK2 cascade cellular response to cAMP cellular response to glucose stimulus response to antineoplastic agent liver regeneration regulation of cell junction assembly cellular response to nerve growth factor stimulus negative regulation of synaptic vesicle exocytosis positive regulation of vasculogenesis small GTPase mediated signal transduction nervous system development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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