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Invitrogen
RAP1A Monoclonal Antibody (5F8)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
RAP1A Antibody (MA5-17164) in WB
Western blot was performed using Anti-RAP1A Monoclonal Antibody (5F8) (Product # MA5-17164) and 20 kDa band corresponding to RAP1A was observed across the cell lines tested except C2C12 in which the expression was observed upon myoblast differentiation. Whole cell lysates (30ug lysate) of C2C12 (Lane 1), C2C12 differentiated to Myoblasts (Lane2), HT-29 (Lane 3), MDA-MB-231 (Lane 4), MCF 10A (Lane 5), A-431 (Lane 6), HeLa (Lane 7) and A-375 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolve... View More
Product Details
MA5-17164
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5F8
Immunogen
Purified recombinant fragment of human RAP1A expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538635
Product Specific Information
MA5-17164 targets RAP1A in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.
The MA5-17164 immunogen is purified recombinant fragment of human RAP1A expressed in E. Coli.
MA5-17164 detects RAP1A which has a predicted molecular weight of approximately 21kDa.
Target Information
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C21KG; G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1; Ras-related protein Rap-1A
Gene Aliases: C21KG; G-22K; KREV-1; KREV1; RAP1; RAP1A; SMGP21
UniProt ID: (Human) P62834
Entrez Gene ID: (Human) 5906
activation of MAPKK activity
signal transduction
positive regulation of neuron projection development
protein transport
microvillus assembly
Rap protein signal transduction
negative regulation of collagen biosynthetic process
cellular response to drug
nerve growth factor signaling pathway
positive regulation of GTPase activity
positive regulation of protein kinase activity
positive regulation of glucose import
positive regulation of phagocytosis
regulation of insulin secretion
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to cAMP
cellular response to glucose stimulus
response to antineoplastic agent
liver regeneration
regulation of cell junction assembly
cellular response to nerve growth factor stimulus
negative regulation of synaptic vesicle exocytosis
positive regulation of vasculogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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