Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
FIGURE: 1 / 2
RASSF1 Antibody (PA5-102383) in IHC (P)
Immunohistochemistry analysis of RASSF1 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RASSF1 polyclonal antibody (Product # PA5-102383) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-102383
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RASSF1(Accession Q9NS23), corresponding to amino acid residues G162-K212.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2851790
Product Specific Information
Antibody detects endogenous levels of total RASSF1.
Target Information
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 123F protein; cardiac-specific ras association domain family 1 protein; pancreas-specific ras association domain family 1 protein; Protein 123F2; Ras association (RalGDS/AF-6) domain family 1; Ras association (RalGDS/AF-6) domain family member 1; Ras association domain family 1; Ras association domain-containing protein 1; RASSF1; RASSF1A tumor suppressor; tumor suppressor protein RDA32; WUGSC:H_LUCA12.5
Gene Aliases: 123F2; AA536941; AU044980; NORE2A; RASSF1; RASSF1A; Rassf1B; Rassf1C; RDA32; REH3P21
UniProt ID: (Human) Q9NS23, (Mouse) Q99MK9
Entrez Gene ID: (Human) 11186, (Mouse) 56289, (Rat) 363140
cell cycle arrest
Ras protein signal transduction
regulation of microtubule cytoskeleton organization
cellular response to DNA damage stimulus
cell cycle
signal transduction
positive regulation of protein ubiquitination
intracellular signal transduction
protein stabilization
negative regulation of cell cycle arrest
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support