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Application
FIGURE: 1 / 1
RASSF1 Antibody (PA5-88305) in WB
Western blot analysis of RASSF1 in extracts of various cell lines. Samples were incubated with RASSF1 Polyclonal antibody (Product # PA5-88305) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-88305
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 101-340 of human RASSF1 (NP_0091133)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.68 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804816
Product Specific Information
Immunogen sequence: CCGPRDLGWE PAVERDTNVD EPVEWETPDL SQAEIEQKIK EYNAQINSNL FMSLNKDGSY TGFIKVQLKL VRPVSVPSSK KPPSLQDARR GPGRGTSVRR RTSFYLPKDA VKHLHVLSRT RAREVIEALL RKFLVVDDPR KFALFERAER HGQVYLRKLL DDEQPLRLRL LAGPSDKALS FVLKENDSGE VNWDAFSMPE LHNFLRILQR EEEEHLRQIL QKYSYCRQKI QEALHACPLG; Positive Samples: HeLa, HepG2, 293T, SW480; Cellular Location: Cytoplasm, Nucleus, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle, spindle pole
Target Information
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiac-specific ras association domain family 1 protein; pancreas-specific ras association domain family 1 protein; Ras association (RalGDS/AF-6) domain family member 1; Ras association domain-containing protein 1; RASSF1; tumor suppressor protein RDA32; WUGSC:H_LUCA12.5
Gene Aliases: 123F2; NORE2A; RASSF1; RASSF1A; RDA32; REH3P21
UniProt ID: (Human) Q9NS23
Entrez Gene ID: (Human) 11186
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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