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Invitrogen

RBL1 Polyclonal Antibody

Advanced Verification

View all (23) RBL1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

Application

RBL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

RBL1 Antibody (PA5-84228) in ICC/IF

Immunofluorecent analysis of RBL1 in human cell line HeLa using RBL1 Polyclonal Antibody (Product # PA5-84228). Staining shows localization to nucleoplasm.

RBL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RBL1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of RBL1 demonstrates antibody specificity. Immunohistochemical analysis of RBL1 using anti-RBL1 Polyclonal Antibody (Product #PA5-84228), shows significant staining of RBL1 in lymph node and shows minimal or weak staining in pancreas tissues. The relative expression levels of RBL1 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-84228

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RBL1. Recombinant protein control fragment (Product #RP-95260).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2791380

Product Specific Information

Immunogen sequence: ESLAWSHDSA LWEALQVSAN KVPTCEEVIF PNNFETGNGG NVQGHLPLMP MSPLMHPRVK EVRTDSGSLR RDMQPLSPIS VHER

Target Information

RB-like 1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and RB-like 1 can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that RB-like 1 may also be a tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 107 kDa retinoblastoma-associated protein; MGC40006; p107; pRb1; retinoblastoma-like 1; Retinoblastoma-like protein 1

Gene Aliases: CP107; p107; PRB1; RBL1

UniProt ID: (Human) P28749

Entrez Gene ID: (Human) 5933

Function(s)

protein binding transcription factor binding promoter-specific chromatin binding chromatin/chromatin-binding, or -regulatory protein

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated cell cycle negative regulation of gene expression viral process covalent chromatin modification regulation of lipid kinase activity positive regulation of transcription from RNA polymerase II promoter regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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