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Invitrogen

RBM10 Polyclonal Antibody

Advanced Verification

View all (11) RBM10 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

Application

RBM10 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

RBM10 Antibody (PA5-83253) in ICC/IF

Immunofluorecent analysis of RBM10 in human cell line A-431 using RBM10 Polyclonal Antibody (Product # PA5-83253). Staining shows localization to nuclear speckles.

RBM10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RBM10 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-549 cells were transfected with, control siRNA, RBM10 siRNA probe #1, and RBM10 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-RBM10 Polyclonal Antibody (Product # PA5-83253) used at a dilution of 0.4 µg/mL. Anti-RBM10 was used as a loading control. {KD}

Product Details

PA5-83253

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RBM10. Recombinant protein control fragment (Product #RP-95471).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2790409

Product Specific Information

Immunogen sequence: MEYERRGGRG DRTGRYGATD RSQDDGGENR SRDHDYRDMD YRSYPREYGS QEGKHDYDDS SEEQSAEDSY EASPGSETQR R

Target Information

The protein encoded by this gene contains RNA recognition motif found in a variety of RNA binding proteins, including various hnRNP proteins, proteins implicated in regulation of alternative splicing, and protein components of snRNPs. In vitro studies showed that the rat homolog bound to RNA homopolymers, with a preference for G and U polyribonucleotides. This gene is part of a gene cluster on chromosome Xp11.23, and its 3' end lies within 20 kb upstream of UBE1. Two transcript variants encoding different isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: G patch domain-containing protein 9; rna-binding; RNA-binding motif protein 10; RNA-binding protein 10; RNA-binding protein S1-1; S1-1

Gene Aliases: DXS8237E; GPATC9; GPATCH9; KIAA0122; RBM10; S1-1; TARPS; ZRANB5

UniProt ID: (Human) P98175

Entrez Gene ID: (Human) 8241

Function(s)

nucleotide binding protein binding zinc ion binding protein complex binding identical protein binding poly(A) RNA binding

Process(es)

regulation of alternative mRNA splicing, via spliceosome mRNA processing biological_process RNA splicing regulation of apoptotic process

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