Loading ...

Invitrogen

RBPJ Polyclonal Antibody

Advanced Verification

View all (26) RBPJ antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (1)

Application

RBPJ Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

RBPJ Antibody (720219) in ICC/IF

For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous CSL/ RBP J using CSL/RBP J Rabbit Polyclonal Antibody (Product # 720219, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CSL/ RBP J protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). ... View More

Product Image Gallery

Antibody specificity was demonstrated by detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using CSL/RBPJ Antibody, Rabbit Polyclonal (Product # 720219) with relevant positive and negative binding sites. {RE}

Product Details

720219

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

ChIP assay (ChIP)

1.5 µg x 10^6 cells

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Protein corresponding to Human RBPJ (aa 1-200)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 30% glycerol

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2633205

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Mouse, Monkey, Bovine, Equine and Pig

Target Information

CSL plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. The protein is abundantly expressed in heart and skeletal muscle and expression is increased by passive stretch. Mutations in this gene can lead to X-linked deafness.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: CBF-1; H-2K binding factor-2; immunoglobulin kappa J region recombination signal binding protein 1; J kappa-recombination signal-binding protein; RBP-J; RBP-J kappa; RBP-JK; rbp-jkappa; Recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; suppressor of hairless homolog

Gene Aliases: AOS3; CBF1; csl; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJ; RBPJK; RBPSUH; SUH

UniProt ID: (Human) Q06330

Entrez Gene ID: (Human) 3516

Function(s)

recombinase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding sequence-specific DNA binding protein N-terminus binding DNA-binding transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter angiogenesis somitogenesis epithelial to mesenchymal transition blood vessel remodeling inflammatory response to antigenic stimulus secondary heart field specification outflow tract morphogenesis endocardium morphogenesis epithelial to mesenchymal transition involved in endocardial cushion formation cardiac left ventricle morphogenesis ventricular trabecula myocardium morphogenesis regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation DNA recombination transcription initiation from RNA polymerase II promoter humoral immune response Notch signaling pathway positive regulation of transcription of Notch receptor target negative regulation of cell proliferation auditory receptor cell fate commitment epidermal cell fate specification pituitary gland development B cell differentiation keratinocyte differentiation negative regulation of ossification positive regulation of BMP signaling pathway somatic stem cell population maintenance dorsal aorta morphogenesis atrioventricular canal development defense response to bacterium myeloid dendritic cell differentiation negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation sebaceous gland development hair follicle maturation positive regulation of cardiac muscle cell proliferation Clara cell differentiation labyrinthine layer blood vessel development arterial endothelial cell fate commitment Notch signaling involved in heart development positive regulation of transcription from RNA polymerase II promoter in response to hypoxia blood vessel lumenization interleukin-4 secretion blood vessel endothelial cell fate specification positive regulation of ERBB signaling pathway positive regulation of ephrin receptor signaling pathway positive regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment positive regulation of cell proliferation involved in heart morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more