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Invitrogen

RETSAT Polyclonal Antibody

5个已发表图片

6 篇参考文献

查看其他3个RETSAT抗体

问题与答案

问题与答案

抗体检测数据 (2)
已发表图片 (5)

Application

RETSAT Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

RETSAT Antibody (PA5-65443) in ICC/IF

Immunofluorescent staining of RETSAT in human cell line U-251 MG shows localization to nucleoli. Samples were probed using a RETSAT Polyclonal Antibody (Product # PA5-65443).

RETSAT Antibody in Immunocytochemistry (ICC/IF)

RETSAT Antibody in Western Blot (WB)

RETSAT Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

RETSAT Antibody in Western Blot, Immunohistochemistry (WB, IHC)

RETSAT Antibody in Western Blot, Immunohistochemistry (WB, IHC)

RETSAT Antibody in Western Blot, Immunoprecipitation (WB, IP)

RETSAT Antibody in Western Blot (WB)

Product Image Gallery

RETSAT Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of RETSAT in human cell line U-251 MG shows localization to nucleoli. Samples were probed using a RETSAT Polyclonal Antibody (Product # PA5-65443).

RETSAT Antibody in Western Blot (WB) — Western blot analysis of RETSAT in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue. Samples were probed using a RETSAT Polyclonal Antibody (Product # PA5-65443).

RETSAT Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 3 RETSAT promotes fork restarting under replication stress. A Co-immunostaining of RETSAT (green) and BrdU pulse labeled replication foci (red) in PANC-1 cells cultured under normoxia (21% O 2 ) or hypoxia (0.3% O 2 ). B iPOND assay to validate location of RETSAT on replication forks. PCNA was included as a positive control. C Immunoblotting of RETSAT in PANC-1 and BxPC-3 infected with or without RETSAT CRISPR gRNA lentivirus. beta actin was used as a loading control. D, E Representative images ( D ) and quantification ( E ) of fork restarting in parental or RETSAT -KO PANC-1 cells under indicated treatments. 2.5 mM Hydroxyurea (HU) was used to induce replication stress. At least 200 single forks were calculated in each sample. F, G Representative images ( F ) and quantification ( G ) of replication fork damage in PANC-1 parental and RETSAT -KO cells under indicated treatments. Pulse labeled BrdU foci was indicating DNA replication sites. gammaH2A.X was used to indicate DNA damage. H, I Immunoblotting of gammaH2A.X in parental and RETSAT -KO PANC-1 ( H ) and BxPC-3 ( I ) under indicated treatments. beta actin was used as a loading control. J Quantification of neutral comet assay in parental and RETSAT -KO PANC-1 cells under indicated treatments. At least 150 single comets were calculated in each sample. K Immunoblotting of ATR, p-ATR (Ser428), CHK1, p-CHK1 (Ser345) andbeta actin in parental and RETSAT -KO PANC-1 cells under indicated treatments. L Flow cytometry based An

RETSAT Antibody in Western Blot, Immunohistochemistry (WB, IHC) — High expression of RETSAT correlated to poor survival in PDAC patients. A The expression of RETSAT in PDAC tumor tissues ( n = 174) from The Cancer Genome Atlas database compare to normal tissues from The Cancer Genome Atlas database and GTEx database ( n = 252). B,C qPCR ( B ) and immunoblotting analysis ( C ) of RETSAT in human pancreatic duct epithelial (HPDE) cell line HPDE6-C7, pancreatic cancer cell lines PANC-1 and BxPC-3, the exposure time was 0.2 s (short exposure) and 1 s (long exposure) respectively in ( C ). D Kaplan-Meier curve for overall survival of PDAC patients ( n = 174) with low vs high expression of RETSAT. The data was downloaded from TCGA dataset and re-analyzed. E Quantification of IHC integrated density of RETSAT in PDAC tissue microarray using image J software. Ninety adjacent tissues and corresponding tumor tissues were calculated. F Classification of PDAC microarray tissues into RETSAT high ( n = 42) and low ( n = 48) subgroups based on the ratio of RETSAT integrated density in tumor tissues versus own adjacent tissues. Tissues with ratio greater than 1.5 were defined as RETSAT-high, while lower than 1.5 were defined as RETSAT-low. G Overall survival time (months) of RETSAT-high and RETSAT-low subgroups based on microarray information. The figure shows the Kaplan-Meier survival curves. H, I Quantification of IHC integrated density of RETSAT in PDAC clinical tissues using image J software ( H ), 80 tumor tissues were calculated and divided into RETSA

RETSAT Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 2 RETSAT deletion sensitized PDAC cells to gemcitabine induced apoptosis. A Representative images of fluorescent Co-immunohistochemistry staining of RETSAT(red) and ductal cell marker CK19 (green) in PDAC tissue microarray. B Co-immunohistofluorescence of RETSAT (red) and HIF-1alpha (green) in PDAC tissue. C, D Immunoblotting ( C ) and quantification ( D ) of RETSAT in PDAC cell lines PANC-1 and BxPC-3. Cells were cultured under normoxia (21% O 2 ) or hypoxia (0.3% O 2 ) for 12, 24, 48, 72 hours respectively. beta actin was used as a loading control. E qPCR analysis of RETSAT transcription under indicated treatments. HIF-1alpha antagonist PX-478(10 muM) was used. F, G Immunoblotting ( F ) and quantification ( G ) of RETSAT in PANC-1 cells under indicated treatments. H Immunoblotting confirmation of RETSAT deletion in luciferase positive PANC-1 cells. I, J Representative images ( I ) and quantification ( J ) of apoptosis using green-fluorescent caspase 3/7 probe labeling in 3D cultured PANC-1 spheroids with or without RETSAT under indicated treatments. K Experimental setup and treatment schedule of CDX assay. Tumor sizes were detected by IVIS system at day 7 post transplantation, and starting Gemcitabine treatment at dose of 50 mg/kg weekly and ending at day 28, the mice were monitored until day 49. L Representative images of bioluminescence signals in mice bearing with PANC-1 cell derived xenografts. M, N Image of cell derived xenografts ( M ) and weight quantification (

RETSAT Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig. 4 RETSAT recruits DDX39B onto replication forks to resolve R-loop. A Treatment and sample collections used for iPOND combined with LC-MS/MS detection. B Overlapping analysis between absent proteins in RETSAT -KO groups and DNA damage response protein dataset. C Heatmap of DDX39B based on its abundance in LC-MS/MS results. D Co-immunoprecipitation using anti-RETSAT antibody combined with immunoblotting to confirm the interaction of RETSAT and DDX39B in PANC-1 cells under indicated treatments. GAPDH was included as a negative control. E Co-immunostaining of DDX39B (green) and pulse labeled BrdU (red) in parental and RETSAT -KO PANC-1 cells. F Immunoblotting of DDX39B in nuclear and cytoplasmic extractions of PANC-1 cells with or without RETSAT. GAPDH and H2B were used as a cytoplasmic and nuclear loading control respectively. G iPOND combined with immunoblotting to examine DDX39B loading dosage on replication forks in parental and RETSAT -KO PANC-1 cells. H, I Representative images ( H ) and quantification ( I ) of R-loop dot blotting using S9.6 antibody in parental and RETSAT -KO PANC-1 cells under indicated treatments. Anti-dsDNA antibody was used as a loading control. J, K Representative images ( J ) and quantification ( K ) of R-loop accumulation in parental and RETSAT -KO PANC-1 cells under indicated treatments. L Dot Blotting of R-loops using S9.6 antibody in parental and RETSAT -KO PANC-1 cells with or without RNase H1 ectopic expression. Anti-dsDNA antibody was use

RETSAT Antibody in Western Blot (WB) — Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

产品信息

PA5-65443

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human RETSAT. Recombinant protein control fragment (Product #RP-104822).

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.40 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2663683

产品详细信息

Immunogen sequence: SSPNPFSEDVK RPPAPLVTDK EARKKVLKQA FSANQVPEKL DVVVIGSGFG GLAAAAILAK AGKRVLVLEQ HTKAGGCCHT FGKNGLEFDT GIHYIGRMEE GSIGRFILDQ ITEGQLDWAP LSSPFDIMVL EGPNGRKEYP M

Highest antigen sequence identity to the following orthologs - mouse 88%, rat 89%.

靶标信息

Retinol saturase carrying out the saturation of the 13-14 double bond of all-trans-retinol to produce all-trans-13,14-dihydroretinol. Has activity toward all-trans-retinol as substrate. Does not use all-trans-retinoic acid nor 9-cis, 11-cis or 13-cis-retinol isomers as substrates. May play a role in the metabolism of vitamin A.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR

Tu Q,Liu X,Yao X,Li R,Liu G,Jiang H,Li K,Chen Q,Huang X,Chang Q,Xu G,Zhu H,Shi P,Zhao B

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Thu Sep 15 00:00:00 UTC 2022

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PLoS genetics

Schleicher EM,Dhoonmoon A,Jackson LM,Clements KE,Stump CL,Nicolae CM,Moldovan GL

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Sun Nov 01 00:00:00 UTC 2020

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PLoS genetics

Schleicher EM,Dhoonmoon A,Jackson LM,Clements KE,Stump CL,Nicolae CM,Moldovan GL

Published figure using RETSAT polyclonal antibody (Product # PA5-65443) in Western Blot

Sun Nov 01 00:00:00 UTC 2020

A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells.

mBio

Chidawanyika T,Mark KMK,Supattapone S

PA5-65443 was used in Western Blotting to show that vitamin playing a role in controlling transport of a small molecule across the cell membrane.

Tue Aug 11 00:00:00 UTC 2020

生物信息学

蛋白别名: All-trans-13,14-dihydroretinol saturase; All-trans-retinol 13,14-reductase; PPAR-alpha-regulated and starvation-induced gene protein; retinol saturase (all-trans-retinol 13,14-reductase); RetSat

基因别名: PPSIG; RETSAT; UNQ439/PRO872

UniProt ID: (Human) Q6NUM9

Entrez Gene ID: (Human) 54884

功能

参与通路

1x1 image pixel for data collection