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Invitrogen
RIG-I Recombinant Rabbit Monoclonal Antibody (35H2L48)
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Application
FIGURE: 1 / 9
RIG-I Antibody (700366) in ICC/IF
Immunofluorescence analysis of RIG-I was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RIG-I Antibody (35H2L48), Recombinant Rabbit Monoclonal (Product # 700366) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa F... View More
Product Details
700366
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
35H2L48
Immunogen
A recombinant protein corresponding to amino acids 454-600 of O95786.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532317
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Retinoic acid-inducible gene I, RIG-I is a pattern recognition receptor (PRR) involved in the recognition of viral dsRNA. Along with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases and they perform overlapping as well as distinct roles. RIG-I is activated by dsRNAs without a 5'-triphosphate end and short dsRNAs, whereas MDA5 is activated by long dsRNAs. Once activated, both proteins signal through IPS-1 activating transcription factors NF-kappaB and IRF-3 (1) and ultimately activating apoptosis, cytokine signaling, and inflammation. RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus (3), paromyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependant antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence has implicated RIG-I in the detection of cytosolic DNA through RNA polymerase III activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Antiviral innate immune response receptor RIG-I; ATP-dependent RNA helicase DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD-box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DEAD/H box polypeptide RIG-I; probable ATP-dependent RNA helicase DDX58; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; retinoic acid-inducible gene-I; RIG-1; RIG-I; RIG-I-like receptor 1; RLR-1; RNA helicase RIG-I; RNA sensor RIG-I
Gene Aliases: 6430573D20Rik; C330021E21; DDX58; RIG-I; RIGI; RLR-1; SGMRT2
UniProt ID: (Human) Q9NT04, (Mouse) Q6Q899
Entrez Gene ID: (Human) 23586, (Mouse) 230073
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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