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RKHD1 Antibody (PA5-95905) in WB
Western blot analysis of RKHD1 in mouse kidney. Samples were incubated with RKHD1 Polyclonal antibody (Product # PA5-95905) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-95905
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human MEX3D (NP_9760493)
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807707
Product Specific Information
Immunogen sequence: AGARRSSGAG TPRHSPTLPE PGGLRLELPL SRRGAPDPVG ALSWRPPQGP VSFPGGAAFS TATSLPSSPA AAACAPLDSG ASENSRKPPS ASSAPALARE C
Target Information
Rkhd1, also known as TINO or MEX3D is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd1, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd1 binds to the AU-rich element in the 3’-untranslated region of Bcl-2 mRNA and is thought to be a negative regulator of Bcl-2 expression. Rkhd3 and Rkhd4, but not Rkhd1, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ring finger (C3HC4 type) and KH domain containing 1; RING finger and KH domain-containing protein 1; RNA-binding protein MEX3D
Gene Aliases: BC059858; Mex3d; Rkhd1
UniProt ID: (Mouse) Q3UE17
Entrez Gene ID: (Mouse) 237400
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