Invitrogen

ROCK1 Monoclonal Antibody (GT464)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (31) ROCK1 antibodies
Datasheet
Protocols
Questions & Answers
Tech Support
Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

MA5-27779

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT464

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735349

Product Specific Information

Positive Control: 293T, HeLa, HepG2, Neuro2A, GL261, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, PC-12 (30uM cisplatin for 24hr)

Predicted Reactivity: Zebrafish (90%), Rabbit (98%), Chicken (96%), Rhesus Monkey (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I

View more View less

Gene Aliases: 1110055K06Rik; P160ROCK; ROCK-I; ROCK1

View more View less

UniProt ID: (Human) Q13464, (Mouse) P70335

View more View less

Entrez Gene ID: (Human) 6093, (Mouse) 19877, (Rat) 81762

View more View less

Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding GTP-Rho binding metal ion binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rho GTPase binding non-receptor serine/threonine protein kinase
Process(es)
apical constriction protein phosphorylation cellular component disassembly involved in execution phase of apoptosis smooth muscle contraction leukocyte cell-cell adhesion signal transduction I-kappaB kinase/NF-kappaB signaling Rho protein signal transduction negative regulation of angiogenesis membrane to membrane docking actin cytoskeleton organization regulation of cell adhesion cortical actin cytoskeleton organization bleb assembly negative regulation of protein binding regulation of actin cytoskeleton organization negative regulation of myosin-light-chain-phosphatase activity negative regulation of neuron apoptotic process regulation of keratinocyte differentiation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway leukocyte migration leukocyte tethering or rolling myoblast migration regulation of stress fiber assembly regulation of focal adhesion assembly positive regulation of focal adhesion assembly establishment of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly regulation of establishment of cell polarity regulation of cell motility apoptotic process cytoskeleton organization phosphorylation regulation of actin filament-based process intracellular signal transduction regulation of cytoskeleton organization
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support