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Invitrogen
RPA2 Monoclonal Antibody (12F3.3)
Application
FIGURE: 1 / 6
RPA2 Antibody (MA1-23284) in ICC/IF
RPA2 Monoclonal Antibody (12F3.3) detects RPA32 protein at DNA damage foci by immunofluorescent analysis. Sample: 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA2 Monoclonal Antibody (12F3.3) (Product # MA1-23284) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
MA1-23284
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
12F3.3
Immunogen
Full-length human RPA-32 expressed in E. coli.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_561100
Product Specific Information
Recommended positive controls: MCF7, T24, Neuro2A, GL261, BCL-1.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 30-kDa protein; HSSB; p32; p34; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP21C; RP4-547C9.3; RpLP1; RpP2
Gene Aliases: AA409079; AI325195; AU020965; REPA2; Rf-A2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34
UniProt ID: (Human) P15927, (Mouse) Q62193
Entrez Gene ID: (Human) 6118, (Mouse) 19891
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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