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Application
FIGURE: 1 / 1
RPH3A Antibody (PA1-4692) in WB
Western blot of RPH3A in rat cortical lysate showing specific immunolabeling of a band at ~82 kDa corresponding to RPH3A polyclonal antibody (Product # PA1-4692).
Product Details
PA1-4692
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C-terminal region of rat RPH3A conjugated to KLH
Conjugate
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_617616
Product Specific Information
This antibody is predicted to react with mouse, human, bovine, canine and non-human primate based on 100% sequence homology.
In Western blot, this antibody is specific for the ~82 kDa rabphilin 3A protein.
Target Information
The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B, and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Exophilin 1; Exophilin-1; Exophilin1; rabphilin 3A homolog; Rabphilin-3A
Gene Aliases: Rph3a
UniProt ID: (Rat) P47709
Entrez Gene ID: (Rat) 171039
transporter activity
calcium ion binding
protein binding
phospholipid binding
calcium-dependent phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
zinc ion binding
selenium binding
Rab GTPase binding
protein complex binding
phosphate ion binding
metal ion binding
inositol 1,4,5 trisphosphate binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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