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Application
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RPS6 Antibody (A300-556A-M) in WB
Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody A300-556A-M (lot A300-556A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A300-556A-M
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 100 and 150 of Human Ribosomal Protein S6.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken and Bovine
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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