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RPS6KA2 Antibody (CF809950) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KA2 (RC201835, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6. Positive lysates LY402841 (100 µg) and LC402841 (20 µg) can be purchased separately from OriGene.
Product Details
CF809950
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3C8
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-51 of human RPS6KA2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
RPS6KA2 (RSK3) serine/threonine kinase is a member of the ribosomal S6 kinase (RSK) family. It is believed that RPS6KA2 is a signal-transducing intermediate in the cellular response to several growth factors. It is found in many tissues, but in higher levels in lung and skeletal muscle. The p90 ribosomal S6 kinases (RSK)1-4 are downstream members of the extracellular signal-regulated kinase (ERK)/MAPK cascade. The loss of RSK2 activity in humans leads to Coffin-Lowry syndrome, which is characterized by mental retardation and growth deficits and may have a tonic regulation on G-protein coupled signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1C; MAPKAPK-1c; mitogen-activated protein kinase-activated protein kinase 1C; p90-RSK 2; p90RSK2; pp90RSK3; Protein-tyrosine kinase Mpk-9; ribosomal protein S6 kinase alpha 2; Ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kD, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 3; RSK-3; S6K-alpha 2; S6K-alpha-2
Gene Aliases: 90kDa; D17Wsu134e; HU-2; MAPKAPK1C; p90-RSK3; p90rsk; p90RSK2; pp90rsk; pp90RSK3; Rps6ka-rs1; RPS6KA2; RSK; RSK3; S6K-alpha; S6K-alpha2
UniProt ID: (Human) Q15349, (Mouse) Q9WUT3
Entrez Gene ID: (Human) 6196, (Mouse) 20112, (Rat) 117269
magnesium ion binding
protein serine/threonine kinase activity
ribosomal protein S6 kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein modifying enzyme
non-receptor serine/threonine protein kinase
oocyte maturation
brain renin-angiotensin system
signal transduction
heart development
negative regulation of cell proliferation
positive regulation of gene expression
cardiac muscle cell apoptotic process
peptidyl-serine phosphorylation
intracellular signal transduction
positive regulation of apoptotic process
negative regulation of cell cycle
negative regulation of meiotic nuclear division
heart contraction
regulation of protein processing
cellular response to carbohydrate stimulus
regulation of translation
protein phosphorylation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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