Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
RSPO3 Antibody (PA5-100324) in ICC/IF
Immunofluorescent analysis of RSPO3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and RSPO3 polyclonal antibody (Product # PA5-100324) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100324
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RSPO3(Accession Q9BXY4), corresponding to amino acid residues F63-K113.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2849838
Product Specific Information
Antibody detects endogenous levels of total RSPO3.
Target Information
RSPO3 is a member of the R-spondin family and plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. RSPO3 has been shown to be involved in regulating the balance between angioblast and blood cell specification during embryonic vasculogenesis and angiogenesis and may be involved in tumor development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Cabriolet; cristin 1; Cristin-1; Cysteine-rich and single thrombospondin domain-containing protein 1; hPWTSR; hRspo3; Nucleopondin; Protein with TSP type-1 repeat; R-spondin 3 homolog; R-spondin 3-like protein; R-spondin-3; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain 2; thrombospondin, type I, domain containing 2
Gene Aliases: 2810459H04Rik; AW742308; CRISTIN1; PWTSR; RGD1563246; RSPO3; THSD2
UniProt ID: (Human) B2RC27, (Mouse) Q2TJ95
Entrez Gene ID: (Human) 84870, (Mouse) 72780, (Rat) 498997
angiogenesis
Wnt signaling pathway
response to stimulus
branching involved in labyrinthine layer morphogenesis
positive regulation of canonical Wnt signaling pathway
biological_process
canonical Wnt signaling pathway
blood vessel remodeling
sprouting angiogenesis
positive regulation of Wnt signaling pathway
positive regulation of non-canonical Wnt signaling pathway
positive regulation of Wnt signaling pathway, planar cell polarity pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support