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Bethyl Laboratories
RUFY1 Polyclonal Antibody
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Application
FIGURE: 1 / 1
RUFY1 Antibody (A304-883A) in IP
Detection of human RUFY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RUFY1 antibody (Product # A304-883A) (lot 1) used for IP at 6 µg per reaction. RUFY1 was also immunoprecipitated by rabbit anti-RUFY1 antibody A304-884A. For blotting immunoprecipitated RUFY1, A304-883A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A304-883A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 275 to 325 of Human RUN and FYVE domain-containing protein 1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; RUN and FYVE domain-containing protein 1; Zinc finger FYVE domain-containing protein 12
Gene Aliases: RABIP4; RUFY1; ZFYVE12
UniProt ID: (Human) Q96T51
Entrez Gene ID: (Human) 80230
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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