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Invitrogen

RUVBL2 Polyclonal Antibody

1 Reference

View all (29) RUVBL2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

Application

RUVBL2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

RUVBL2 Antibody (PA5-29871) in ICC/IF

Immunocytochemistry analysis of RUVBL2 in paraformaldehyde-fixed A549. Samples were incubated with RUVBL2 polyclonal antibody (Product # PA5-29871) using a dilution of 1:200.

RUVBL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RUVBL2 Antibody in Immunoprecipitation (IP)

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Product Details

PA5-29871

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RUVBL2. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.89 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547345

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (91%), Xenopus laevis (96%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination.

Molecular cell

Clarke TL,Sanchez-Bailon MP,Chiang K,Reynolds JJ,Herrero-Ruiz J,Bandeiras TM,Matias PM,Maslen SL,Skehel JM,Stewart GS,Davies CC

PA529871 was used in western blot to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair

Thu Mar 02 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; p47 protein; Repressing pontin 52; reptin; Reptin 52; reptin52 protein; RuvB (E coli homolog)-like 2; RuvB-like 2; RUVBL2 protein; TAP54-beta; TIP49b; TIP60-associated protein 54-beta

Gene Aliases: CGI-46; ECP-51; ECP51; INO80J; mp47; p47; REPTIN; RUVBL2; RVB2; TAP54-beta; TIH2; TIP48; TIP49B

UniProt ID: (Human) Q9Y230, (Mouse) Q9WTM5

Entrez Gene ID: (Human) 10856, (Mouse) 20174

Function(s)

RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding TFIID-class transcription factor binding DNA helicase activity ATP-dependent DNA helicase activity protein binding ATP binding ATPase activity chromatin DNA binding identical protein binding protein homodimerization activity ATP-dependent 5'-3' DNA helicase activity ADP binding unfolded protein binding ATPase binding nucleotide binding damaged DNA binding helicase activity hydrolase activity

Process(es)

DNA repair DNA recombination chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus chromatin modification DNA duplex unwinding cellular response to UV positive regulation of histone acetylation histone H4 acetylation histone H2A acetylation positive regulation of transcription from RNA polymerase II promoter establishment of protein localization to chromatin cellular response to estradiol stimulus transcriptional activation by promoter-enhancer looping negative regulation of estrogen receptor binding box C/D snoRNP assembly protein folding regulation of growth positive regulation of telomerase RNA localization to Cajal body

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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