Loading ...

OriGene

RUVBL2 Monoclonal Antibody (OTI1H10), TrueMAB™

View all (29) RUVBL2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (13)

Application

RUVBL2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

RUVBL2 Antibody (CF504284) in ICC/IF

Anti-RUVBL2 mouse monoclonal antibody (TA504284) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2(RC200933).

RUVBL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RUVBL2 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

CF504284

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1H10

Immunogen

Human recombinant protein fragment corresponding to amino acids 113-370 of human RUVBL2 produced in E.coli.

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; Repressing pontin 52; Reptin 52; reptin52 protein; RuvB (E coli homolog)-like 2; RuvB-like 2; RUVBL2 protein; TAP54-beta; TIP49b; TIP60-associated protein 54-beta

Gene Aliases: CGI-46; ECP-51; ECP51; INO80J; REPTIN; RUVBL2; RVB2; TAP54-beta; TIH2; TIP48; TIP49B

UniProt ID: (Human) Q9Y230

Entrez Gene ID: (Human) 10856

Function(s)

RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding TFIID-class transcription factor binding DNA helicase activity ATP-dependent DNA helicase activity protein binding ATP binding ATPase activity chromatin DNA binding identical protein binding protein homodimerization activity ATP-dependent 5'-3' DNA helicase activity ADP binding unfolded protein binding ATPase binding

Process(es)

box C/D snoRNP assembly DNA repair DNA recombination chromatin remodeling transcription, DNA-templated protein folding DNA duplex unwinding cellular response to UV positive regulation of histone acetylation regulation of growth histone H4 acetylation histone H2A acetylation positive regulation of transcription from RNA polymerase II promoter establishment of protein localization to chromatin cellular response to estradiol stimulus transcriptional activation by promoter-enhancer looping negative regulation of estrogen receptor binding positive regulation of telomerase RNA localization to Cajal body

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more