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Application
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Rabex-5 Antibody (PA5-148258) in ICC/IF
Immunocytochemistry analysis of Rabex-5 in U251MG cells. Samples were incubated with Rabex-5 polyclonal antibody (Product # PA5-148258) using a dilution of 1:200 at 4°C overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 10% serum. Counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Product Details
PA5-148258
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human RABGEF1 fragment
Conjugate
Unconjugated
Form
Liquid
Concentration
100 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3247427
Target Information
RABGEF1 forms a complex with rabaptin-5 (RABPT5) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RAB guanine nucleotide exchange factor (GEF) 1; Rab5 GDP/GTP exchange factor; Rabaptin-5-associated exchange factor for Rab5; Rabex-5; RAP1
Gene Aliases: rabex-5; RABEX5; RABGEF1; RAP1
UniProt ID: (Human) Q9UJ41
Entrez Gene ID: (Human) 27342
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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