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Invitrogen
Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
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Application
FIGURE: 1 / 2
Rat IgG2a kappa Isotype Control (16-4321-82) in IHC (P)
Reduced arthritis progression in KO-Rag−/− mice.The CD11c-Flip-KO mice were crossed with Rag−/− mice to generate the KO-Rag−/− line. (a) The spontaneous development of arthritis in KO-Rag−/−mice was compared with the littermate KO-Rag+/− mice (n=7-10 KO-Rag+/− mice and 7-16 KO-Rag−/− at the observed time points). No arthritis was observed in the Rag−/−or Rag+/− mice. (b) Representative (of four to five per ... View More
Product Details
16-4321-82
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Functional Assay (Functional)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBR2a
Conjugate
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_470156
Product Specific Information
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA.
Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at the same concentration as the experimental antibody.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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