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Application
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RelB Antibody (A302-183A) in WB
Detection of human and mouse RelB by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from Jurkat (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RelB antibody (Product # A302-183A) used for WB at 0.4 µg/mL (A) and 1 µg/mL (B) and used for IP at 10 µg/mg lysate. RelB was also immunoprecipitated by rabbit anti-RelB antibody A302-182A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure... View More
Product Details
A302-183A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 529 and 579 of Human v-rel reticuloendotheliosis viral oncogene homolog B.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The NFkB transcription factor was originally identified as a protein complex consisting of a DNA-binding subunit and an associated protein. The DNAbinding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A second protein designated p52 (previously referred to as p49) has been identified that can act as an alternative NFkB subunit. Rel B does not bind with high affinity to NFkB sites, but heterodimers between Rel B and p50 bind with an affinity comparable to that of p50 NFkB homodimers. However, Rel B/p50 heterodimers, in contrast to NFkB heterodimers, transactivates transcription of promotors containing kB binding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I-Rel; OTTHUMP00000221313; rel b; Transcription factor RelB; v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3); v-rel reticuloen; v-rel reticuloendotheliosis viral oncogene homolog B, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Gene Aliases: I-REL; IREL; REL-B; RELB; shep
UniProt ID: (Human) Q01201, (Mouse) Q04863
Entrez Gene ID: (Human) 5971, (Mouse) 19698
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
stimulatory C-type lectin receptor signaling pathway
transcription, DNA-templated
inflammatory response
I-kappaB kinase/NF-kappaB signaling
antigen processing and presentation
negative regulation of interferon-beta production
circadian regulation of gene expression
response to cytokine
NIK/NF-kappaB signaling
myeloid dendritic cell differentiation
T-helper 1 cell differentiation
innate immune response
positive regulation of transcription from RNA polymerase II promoter
cellular response to osmotic stress
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of gene expression
T-helper 1 type immune response
negative regulation of transcription, DNA-templated
rhythmic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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