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Rockland
Ribonuclease A Polyclonal Antibody, Biotin
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Application
FIGURE: 1 / 1
Ribonuclease A Antibody (200-4688-0100) in WB
Western Blot of Rabbit anti-Ribonuclease A Antibody Biotin Conjugated. Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A. Other band(s): Possible dimerization.
Product Details
200-4688-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Ribonuclease A [Bovine Pancreas]
Conjugate
Biotin
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Ribonuclease A antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas]. Cross reactivity against Ribonuclease A from other sources may occur but have not been specifically determined.
Target Information
Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Rib 1; ribonuclease A (pancreatic); ribonuclease A C1; Ribonuclease BS-1; Ribonuclease pancreatic; RNase 1; RNase A; RNAseA; S-RNase; Seminal ribonuclease; seminal RNase
Gene Aliases: RAC1; RNASE1; RNS1; SRN
UniProt ID: (Bovine) P00669, (Bovine) P61823
Entrez Gene ID: (Bovine) 280930, (Bovine) 282340
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