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Invitrogen
Ribosome-inactivating protein Polyclonal Antibody
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Application
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Ribosome-inactivating protein Antibody (PA5-98352) in WB
Western Blot analysis of Ribosome-inactivating protein using a Ribosome-inactivating protein Polyclonal antibody (Product # PA5-98352) at a concentration of 4 µg/mL. Positive WB detected in: Cucurbita moschata (40µg, 20µg, 10µg, 5µg). A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 28 kDa.
Product Details
PA5-98352
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Cucurbita moschata Ribosome-inactivating protein cucurmosin protein (1-244AA)
Conjugate
Unconjugated
Form
Liquid
Concentration
5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2812965
Target Information
Has cytotoxic activity towards cancer cells, but not normal cells. Inhibits the growth of the human leukemia cell line K562, the murine melanoma cell line B16 and the lung adenocarcinoma cell line A549 with IC50 values of 88.1 nM, 63.4 nM and 359.3 nM respectively. [Uniprot]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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