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Application
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Ric-8A Antibody (A304-572A-T) in WB
Detection of human and mouse Ric-8A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Ric-8A antibody (Product # A304-572A) (lot A304-572A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A304-572A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 418 to 468 of Human Resistance to Inhibitors of Cholinesterase 8 Homolog A.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Orangutan
Target Information
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chaperone Ric-8A; MGC104517; MGC131931; MGC148073; MGC148074; resistance to inhibitors of cholinesterase 8 homolog A; RIC8 protein; synembryn; Synembryn-A
Gene Aliases: AI114950; RIC-8; RIC8; RIC8A
UniProt ID: (Human) Q9NPQ8, (Mouse) Q3TIR3
Entrez Gene ID: (Human) 60626, (Mouse) 101489
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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