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Rubicon Polyclonal Antibody

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Antibody Testing Data (11)

Rubicon Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

Rubicon Antibody (21444-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

Product Image Gallery

Product Details

21444-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Rubicon Fusion Protein Ag13715 (61-290 aa encoded by BC033615)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: VAELWLQHS LQYHCLSAQL RPLLGDRQYI RKFYTDAAFL LSDAHVTAML QCLEAVEQNN PRLLAQIDAS MFARKHESPL LVTKSQSLTA LPSSTYTPPN SYAQHSYFGS FSSLHQSVPN NGSERRSTSF PLSGPPRKPQ ESRGHVSPAE DQTIQAPPVS VSALARDSPL TPNEMSSSTL TSPIEASWVS SQNDSPGDAS EGPEYLAIGN LDPRGRTASC QSHSSNAESS S (61-290 aa encoded by BC033615)

Target Information

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rubicon can predict prognosis in patients with pancreatic ductal adenocarcinoma after neoadjuvant chemoradiotherapy.

International journal of clinical oncology

Marukawa D,Gotoh K,Kobayashi S,Sasaki K,Iwagami Y,Yamada D,Tomimaru Y,Akita H,Asaoka T,Noda T,Takahashi H,Tanemura M,Doki Y,Eguchi H

Sat Apr 01 00:00:00 EDT 2023

Rubicon can predict prognosis in patients with pancreatic ductal adenocarcinoma after neoadjuvant chemoradiotherapy.

International journal of clinical oncology

Marukawa D,Gotoh K,Kobayashi S,Sasaki K,Iwagami Y,Yamada D,Tomimaru Y,Akita H,Asaoka T,Noda T,Takahashi H,Tanemura M,Doki Y,Eguchi H

Sat Apr 01 00:00:00 EDT 2023

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Li T,Su X,Lu P,Kang X,Hu M,Li C,Wang S,Lu D,Shen S,Huang H,Liu Y,Deng X,Cai W,Wei L,Lu Z

Tue Aug 01 00:00:00 EDT 2023

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Li T,Su X,Lu P,Kang X,Hu M,Li C,Wang S,Lu D,Shen S,Huang H,Liu Y,Deng X,Cai W,Wei L,Lu Z

Tue Aug 01 00:00:00 EDT 2023

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Li T,Su X,Lu P,Kang X,Hu M,Li C,Wang S,Lu D,Shen S,Huang H,Liu Y,Deng X,Cai W,Wei L,Lu Z

Tue Aug 01 00:00:00 EDT 2023

Inhibition of interaction between ROCK1 and Rubicon restores autophagy in endothelial cells and attenuates brain injury after prolonged ischemia.

Journal of neurochemistry

Lin Y,Zhan Z,Hu M,Li H,Zhang B,Wu R,Tan S,Shan Y,Lu Z,Qin B

Sun Jan 01 00:00:00 EST 2023

Inhibition of interaction between ROCK1 and Rubicon restores autophagy in endothelial cells and attenuates brain injury after prolonged ischemia.

Journal of neurochemistry

Lin Y,Zhan Z,Hu M,Li H,Zhang B,Wu R,Tan S,Shan Y,Lu Z,Qin B

Sun Jan 01 00:00:00 EST 2023

FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation.

Autophagy

Cai W,Hu M,Li C,Wu R,Lu D,Xie C,Zhang W,Li T,Shen S,Huang H,Qiu W,Liu Q,Lu Y,Lu Z

Sat Apr 01 00:00:00 EDT 2023

FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation.

Autophagy

Cai W,Hu M,Li C,Wu R,Lu D,Xie C,Zhang W,Li T,Shen S,Huang H,Qiu W,Liu Q,Lu Y,Lu Z

Sat Apr 01 00:00:00 EDT 2023

FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation.

Autophagy

Cai W,Hu M,Li C,Wu R,Lu D,Xie C,Zhang W,Li T,Shen S,Huang H,Qiu W,Liu Q,Lu Y,Lu Z

Sat Apr 01 00:00:00 EDT 2023

Rubicon-regulated beta-1 adrenergic receptor recycling protects the heart from pressure overload.

Scientific reports

Akazawa Y,Taneike M,Ueda H,Kitazume-Taneike R,Murakawa T,Sugihara R,Yorifuji H,Nishida H,Mine K,Hioki A,Omiya S,Nakayama H,Yamaguchi O,Yoshimori T,Sakata Y,Otsu K

Fri Jan 07 00:00:00 EST 2022

Phosphorylated STYK1 restrains the inhibitory role of EGFR in autophagy initiation and EGFR-TKIs sensitivity.

Cell insight

Zhou C,Dong X,Wang M,Qian X,Hu M,Liang K,Liang Y,Zhang R,Huang Y,Lyu H,Xiao S,Tang Y,Ali DW,Michalak M,Chen XZ,Tang J

Mon Aug 01 00:00:00 EDT 2022

Dickkopf1 (Dkk1) Alleviates Vascular Calcification by Regulating the Degradation of Phospholipase D1 (PLD1).

Journal of cardiovascular translational research

Li X,Liu XL,Li X,Zhao YC,Wang QQ,Zhong HY,Liu DD,Yuan C,Zheng TF,Zhang M

Thu Dec 01 00:00:00 EST 2022

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.

ACS nano

Shen S,Cheng X,Zhou L,Zhao Y,Wang H,Zhang J,Sun X,Wang Y,Shu Y,Xu Y,Tao Y,Li M,Lu Z,Cai W,Nie G,Qiu W

Tue Nov 22 00:00:00 EST 2022

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.

ACS nano

Shen S,Cheng X,Zhou L,Zhao Y,Wang H,Zhang J,Sun X,Wang Y,Shu Y,Xu Y,Tao Y,Li M,Lu Z,Cai W,Nie G,Qiu W

Tue Nov 22 00:00:00 EST 2022

17β-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis.

International journal of biological sciences

Sun Z,Qu J,Xia X,Pan Y,Liu X,Liang H,Dou H,Hou Y

Fri Jan 14 00:00:00 EST 2022

ZKSCAN3 in severe bacterial lung infection and sepsis-induced immunosuppression.

Laboratory investigation; a journal of technical methods and pathology

Ouyang X,Becker E,Bone NB,Johnson MS,Craver J,Zong WX,Darley-Usmar VM,Zmijewski JW,Zhang J

Mon Nov 01 00:00:00 EDT 2021

STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Autophagy

Zhou C,Qian X,Hu M,Zhang R,Liu N,Huang Y,Yang J,Zhang J,Bai H,Yang Y,Wang Y,Ali D,Michalak M,Chen XZ,Tang J

Thu Oct 01 00:00:00 EDT 2020

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Autophagy

Huang X,Gan G,Wang X,Xu T,Xie W

Mon Jul 01 00:00:00 EDT 2019

Inducible Rubicon facilitates viral replication by antagonizing interferon production.

Cellular & molecular immunology

Wan Y,Cao W,Han T,Ren S,Feng J,Chen T,Wang J,Broering R,Lu M,Zhu Y

Sat Jul 01 00:00:00 EDT 2017

Inducible Rubicon facilitates viral replication by antagonizing interferon production.

Cellular & molecular immunology

Wan Y,Cao W,Han T,Ren S,Feng J,Chen T,Wang J,Broering R,Lu M,Zhu Y

Sat Jul 01 00:00:00 EDT 2017

Inducible Rubicon facilitates viral replication by antagonizing interferon production.

Cellular & molecular immunology

Wan Y,Cao W,Han T,Ren S,Feng J,Chen T,Wang J,Broering R,Lu M,Zhu Y

Sat Jul 01 00:00:00 EDT 2017

Inducible Rubicon facilitates viral replication by antagonizing interferon production.

Cellular & molecular immunology

Wan Y,Cao W,Han T,Ren S,Feng J,Chen T,Wang J,Broering R,Lu M,Zhu Y

Sat Jul 01 00:00:00 EDT 2017

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Autophagy

Muniz-Feliciano L,Doggett TA,Zhou Z,Ferguson TA

Fri Jul 05 00:00:00 EDT 2019

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Autophagy

Muniz-Feliciano L,Doggett TA,Zhou Z,Ferguson TA

Fri Jul 05 00:00:00 EDT 2019

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Autophagy

Muniz-Feliciano L,Doggett TA,Zhou Z,Ferguson TA

Fri Jul 05 00:00:00 EDT 2019

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Autophagy

Muniz-Feliciano L,Doggett TA,Zhou Z,Ferguson TA

Fri Jul 05 00:00:00 EDT 2019

USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.

Genes & development

Xu D,Shan B,Sun H,Xiao J,Zhu K,Xie X,Li X,Liang W,Lu X,Qian L,Yuan J

Mon Aug 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Baron; Beclin-1 associated RUN domain containing protein; Rubicon; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1 interacting and cysteine-rich containing protein; Run domain Beclin-1-interacting and cysteine-rich domain-containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; rundataxin; similar to mKIAA0226 protein

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Gene Aliases: 5330403K09; KIAA0226; mKIAA0226; RUBCN; RUBICON; SCAR15

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UniProt ID: (Mouse) Q80U62, (Human) Q92622

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Entrez Gene ID: (Mouse) 100502698, (Rat) 303885, (Human) 9711

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Function(s)

molecular_function protein binding

Process(es)

biological_process negative regulation of autophagy negative regulation of endocytosis immune system process phagocytosis negative regulation of phosphatidylinositol 3-kinase activity multivesicular body sorting pathway autophagosome maturation negative regulation of autophagosome maturation endocytosis autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Rubicon Polyclonal Antibody

Rubicon Antibody (21444-1-AP) in IHC (P)

Product Details

21444-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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