Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Special Offers
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Primary Antibodies
  • Search All
  • Popular Product Areas
  • TaqMan Assays
  • Primary Antibodies
  • Secondary Antibodies
  • Isotype Controls
  • Proteins & Peptides
  • ELISA Kits
  • All Documents & Support
  • Certificates
  • SDS
  • Manuals & Protocols
  • Product FAQs
    Search button Close
            • Order Status
            • Quick Order
            • Sign in
              Sign in
              Don't have an account ? Create Account
              • Account
              • Check Order Status
              • Aspire Member Program
              • Connect: Lab, Data, Apps
              • Custom Products & Projects
              • Services Central

            Disclaimer

            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

            Loading ...
            • Primary Antibodies ›
            • S100 Antibodies

            Invitrogen

            S100 Monoclonal Antibody (4C4.9)

            3 Published Figures
            70 References
            View all (13) S100 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite S100 Monoclonal Antibody (4C4.9)

            • Antibody Testing Data (4)
            • Published Figures (3)
            Application
            S100 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 7

            S100 Antibody (MA5-12969) in ICC/IF

            Immunofluorescent analysis of S100 Protein (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a S100 Protein monoclonal antibody (Product # MA5-12969) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a D... View More
            View Product

            S100 Antibody in Immunocytochemistry (ICC/IF)
            S100 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            S100 Antibody in Western Blot (WB)
            S100 Antibody in Western Blot (WB)
            S100 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)
            S100 Antibody in Immunohistochemistry (IHC)
            S100 Antibody in Immunohistochemistry (IHC)

            Product Details

            MA5-12969

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:100-1:1,000
            View 3 publications 3 publications

            Immunohistochemistry (IHC)

            -
            View 56 publications 56 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:100
            View 4 publications 4 publications

            Immunocytochemistry (ICC/IF)

            1:10-1:100
            View 3 publications 3 publications

            Flow Cytometry (Flow)

            -
            View 1 publication 1 publication

            Miscellaneous PubMed (Misc)

            -
            View 3 publications 3 publications
            Product Specifications

            Species Reactivity

            Bovine, Human, Mouse

            Published species

            Human, Mouse, Non-human primate, Rat

            Host/Isotype

            Mouse / IgG2a, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            4C4.9

            Immunogen

            Purified bovine brain S100 protein
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Protein A/G

            Storage buffer

            PBS, pH 7.4, with 0.05% BSA

            Contains

            0.05% sodium azide

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_10983883

            Product Specific Information

            MA5-12969 targets S100 Protein in IHC (P), ICC/IF and WB applications and shows reactivity with Bovine, Human, mouse, and Rat samples.

            The MA5-12969 immunogen is purified bovine brain S100 protein.

            Target Information

            S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: Protein S100-A1; Protein S100-B; S 100; S-100 calcium-binding protein, beta chain; S-100 protein alpha chain; S-100 protein beta chain; S-100 protein subunit alpha; S-100 protein subunit beta; S100 alpha; S100 calcium-binding protein A1; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S100 protein, alpha polypeptide; S100 protein, beta polypeptide; S100alpha

            View more View less

            Gene Aliases: AI266795; AI850290; Bpb; NEF; S100; S100-alpha; S100-B; S100A; S100A1; S100B; S100beta

            View more View less

            UniProt ID: (Bovine) P02639, (Human) P23297, (Human) P04271, (Bovine) A4IFR6, (Mouse) P56565, (Mouse) P50114

            View more View less

            Entrez Gene ID: (Bovine) 528735, (Human) 6271, (Human) 6285, (Bovine) 525716, (Mouse) 20193, (Mouse) 20203

            View more View less

            Function(s)

            calcium ion binding protein binding identical protein binding protein homodimerization activity S100 protein binding calcium-dependent protein binding ATPase binding zinc ion binding tau protein binding RAGE receptor binding kinase inhibitor activity metal ion binding receptor binding calmodulin-related

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter regulation of heart contraction substantia nigra development intracellular signal transduction positive regulation of voltage-gated calcium channel activity axonogenesis central nervous system development learning or memory memory cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of neuronal synaptic plasticity astrocyte differentiation response to glucocorticoid response to methylmercury long-term synaptic potentiation cellular response to hypoxia negative regulation of skeletal muscle cell differentiation regulation of translation phosphorylation negative regulation of kinase activity positive regulation of complement activation astrocyte activation negative regulation of monocyte chemotactic protein-1 production energy reserve metabolic process cellular calcium ion homeostasis regulation of cytokine biosynthetic process regulation of long-term neuronal synaptic plasticity positive regulation of synaptic transmission
            It has to be done as per old AB suggested Products section.
            guarantee_icon

            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

            Learn more
            help_icon

            We're here to help

            Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

            Contact tech support
            Ordering Plus Icon Minus Icon
            • Order Status
            • Order Help
            • Quick Order
            • Supply Center
            • eProcurement
            Support Plus Icon Minus Icon
            • Help and Support
            • Contact Us
            • Technical Support Centers
            • Documents and Certificates
            • Report a Site Issue
            Resources Plus Icon Minus Icon
            • Learning Centers
            • Promotions
            • Events and Webinars
            • Social Media
            About Thermo Fisher Plus Icon Minus Icon
            • About Us
            • Careers
            • Investors
            • News
            • Social Responsibility
            • Trademarks
            • Consumer Health Data Privacy Policy
            Our Portfolio Plus Icon Minus Icon
            • Thermo Scientific
            • Applied Biosystems
            • Invitrogen
            • Gibco
            • Ion Torrent
            • Fisher Scientific
            • Unity Lab Services
            • Patheon
            • PPD
            • Terms & Conditions
            • Privacy Information Center
            • Price & Freight Policy
            • Cookie Preferences

              Your choices regarding cookies on this site

              We and our affiliates and vendors use cookies and similar technologies to operate our sites, recognize visitors to our sites, provide secure log-in, collect statistics to optimize site functionality, and deliver content tailored to your interests. Click Accept All to accept all cookies and go directly to the site, click Reject All to reject all but cookies strictly necessary to the functioning of this site (required cookies), or click on Manage Settings to see detailed descriptions of the types of cookies and choose whether to accept certain cookies while on the site.
            © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
            United States flag icon
            United States

            Your items have has been added!


            Host server : magellan-search-green-56756dfb8b-fsh6n:80/100.66.78.219:80.
            git-commit: de6905f249d25e22729e613aef254bb544bd8b7c
            git-url: https://github.com/thermofisher/magellan-search
            git-branch: release/2.31.1-2025.06.25-1.0