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Invitrogen
S100B Monoclonal Antibody (SH-B4)
Application
FIGURE: 1 / 11
S100B Antibody (MA1-25005) in ICC/IF
Immunofluorescence analysis of Protein S100-B was performed using 70% confluent log phase SK-N-MC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with S100B Monoclonal Antibody (SH-B4) (Product # MA1-25005) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488... View More
Product Details
MA1-25005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Cat,
Goat,
Human,
Mouse,
Sheep,
Pig,
Rabbit,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SH-B4
Immunogen
Bovine brain S-100b.
Conjugate
Unconjugated
Form
Liquid
Concentration
11.5 mg/mL
Storage buffer
Ascites
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_795382
Product Specific Information
Recommended positive controls: human tongue.
MA1-25005 has a predicted molecular weight of 10.5 kDa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Protein S100-B; S-100 calcium-binding protein beta subunit; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein beta (neural); S100 calcium-binding protein, beta (neural); S100 protein beta chain; S100 protein, beta polypeptide; S100 protein, beta polypeptide, neural
Gene Aliases: AI850290; Bpb; NEF; S100; S100-B; S100B; S100beta; S100P
UniProt ID: (Human) P04271, (Bovine) A4IFR6, (Rabbit) Q6YNR6, (Mouse) P50114, (Rat) P04631
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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