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SARS-CoV-2 ORF9b Antibody (PA5-143833) in ELISA
ELISA analysis of SARS-CoV-2 ORF9b. Samples were incubated with SARS-CoV-2 ORF9b Polyclonal antibody (Product # PA5-143833) followed by Goat anti-rabbit IgG HRP conjugate secondary antibody at a dilution of 1:20,000. A sandwich ELISA was performed using immunogen peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9b antibody as the capture antibody. Detection range is from 0.3 ng/mL to 1,000 ng/mL.
Product Details
PA5-143833
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to 14 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) ORF9b protein. The immunogen is located in the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9b protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3075047
Product Specific Information
Predicted reactivity based on immunogen sequence: SARS-CoV ORF9b proteins: (50%)
Target Information
ORF9b plays a role in the inhibition of host innate immune response by targeting the mitochondrial-associated adapter MAVS. Mechanistically, usurps the E3 ligase ITCH to trigger the degradation of MAVS, TRAF3, and TRAF6. In addition, causes mitochondrial elongation by triggering ubiquitination and proteasomal degradation of dynamin-like protein 1/DNM1L.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: COVID-19 ORF9b; COVID19 ORF9b; ORF9b; SARS-CoV-2 Open Reading Frame 9b; SARS-COV2 ORF9b
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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