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Invitrogen
SARS-CoV-2 Spike Protein (S1/S2) Recombinant Rabbit Monoclonal Antibody (ARC2373)
FIGURE: 1 / 1
SARS-CoV-2 Spike Protein (S1/S2) Antibody (MA5-36087) in WB
Western blot analysis of SARS-CoV-2 Spike Protein (S1/S2) in extracts from control 293T and SARS-CoV-2 Spike S1-293T transfected cells. Samples were incubated with SARS-CoV-2 Spike Protein (S1/S2) Monoclonal antibody (Product # MA5-36087) using a dilution of 1:10,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk i... View More
Product Details
MA5-36087
Applications
Tested Dilution
Publications
Product Specifications
Published species
Hamster
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2373
Immunogen
Recombinant fusion protein of SARS-CoV-2 Spike S1.
Conjugate
Form
Liquid
Concentration
2.28 mg/mL
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_2866698
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. SARS-CoV-2 S is 1273 amino acids long and consists of a signal peptide (amino acids 1-13) located at the N-terminus, the S1 subunit (14-685 residues), and the S2 subunit (686-1273 residues); the last two regions are responsible for receptor binding and membrane fusion, respectively. The S-ECD is the extracellular domain of the Spike protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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