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Application
FIGURE: 1 / 3
SCRIB Antibody (PA5-66680) in ICC/IF
Immunofluorescence analysis of SCRIB was performed using MCF7 and Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SCRIB Polyclonal Antibody (Product # PA5-66680) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2... View More
Product Details
PA5-66680
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human SCRIB. Recombinant protein control fragment (Product #RP-107328).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.36 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2663281
Product Specific Information
Immunogen sequence: RLLEVNQQSLL GLTHGEAVQL LRSVGDTLTV LVCDGFEAST DAALEVSPGV IANPFAAGIG HRNSLESISS IDRE
Highest antigen sequence identity to the following orthologs - mouse 92%, rat 92%.
Target Information
SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hScrib; Protein LAP4; Protein scribble homolog; S Vartul; Scribble; scribbled homolog
Gene Aliases: CRIB1; KIAA0147; LAP4; SCRB1; SCRIB; SCRIB1; VARTUL
UniProt ID: (Human) Q14160
Entrez Gene ID: (Human) 23513
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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