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Application
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SEMA5A Antibody (PA5-31283) in WB
Western Blot using SEMA5A Polyclonal Antibody (Product # PA5-31283). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 5% SDS PAGE. SEMA5A Polyclonal Antibody (Product # PA5-31283) diluted at 1:1,000.
Product Details
PA5-31283
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 700 and 1000 of Human Semaphorin 5A
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548757
Product Specific Information
Recommended positive controls: Mouse brain.
Predicted reactivity: Mouse (95%), Rat (94%), Rhesus Monkey (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development (Adams et al., 1996 [PubMed 8817451]).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: M-Sema D; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; Sema F; semaphorin F; Semaphorin-5A; Semaphorin-F
Gene Aliases: 5930434A13; 9130201M22Rik; AI464145; SEMA5A; SEMAF; semF
UniProt ID: (Human) Q13591
Entrez Gene ID: (Human) 9037, (Mouse) 20356
neural crest cell migration
positive regulation of endothelial cell proliferation
blood vessel endothelial cell proliferation involved in sprouting angiogenesis
cell adhesion
negative regulation of cell adhesion
cell-cell signaling
nervous system development
axonal fasciculation
diencephalon development
positive regulation of cell migration
positive regulation of actin filament depolymerization
positive regulation of catenin import into nucleus
positive regulation of angiogenesis
positive regulation of axon extension involved in axon guidance
negative regulation of axon extension involved in axon guidance
positive chemotaxis
negative chemotaxis
positive regulation of protein kinase B signaling
cell chemotaxis
semaphorin-plexin signaling pathway
signal clustering
negative regulation of endothelial cell apoptotic process
positive regulation of endothelial cell chemotaxis
patterning of blood vessels
multicellular organism development
axon guidance
cell differentiation
branching morphogenesis of an epithelial tube
positive regulation of extracellular matrix disassembly
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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