Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
SERCA1 Antibody (ACP-011-200UL) in ICC/IF
Expression of SERCA1 in mouse C2C12 myoblast cell line - Immunocytochemical staining of fixed and permeabilized C2C12 cells. A. Cells were stained with Anti-SERCA1 Antibody (#ACP-011), (1:400),followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live image of the cells stained in A. C. Merge image of A and B and visualization of cell nuclei using Hoechst 33342 (blue).
Product Details
ACP-011-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)TPDQVKRHLEKYG, corresponding to amino acid residues 25-37 of rat SERCA1, Cytoplasmic, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA; SERCA ATPase; SERCA1; SR Ca(2+)-ATPase 1
Gene Aliases: Atp2a1; SERCA1
UniProt ID: (Mouse) Q8R429, (Rat) Q64578
Entrez Gene ID: (Mouse) 11937, (Rat) 116601
transport
ion transport
calcium ion transport
regulation of muscle contraction
regulation of striated muscle contraction
apoptotic mitochondrial changes
positive regulation of fast-twitch skeletal muscle fiber contraction
positive regulation of endoplasmic reticulum calcium ion concentration
negative regulation of endoplasmic reticulum calcium ion concentration
response to endoplasmic reticulum stress
response to peptide hormone
negative regulation of striated muscle contraction
positive regulation of mitochondrial calcium ion concentration
maintenance of mitochondrion location
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
sarcoplasmic reticulum calcium ion transport
calcium ion import
calcium ion transmembrane transport
relaxation of skeletal muscle
carbohydrate derivative transport
nucleotide transmembrane transport
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support