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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • SERPINH1 Antibodies

            Invitrogen

            SERPINH1 Monoclonal Antibody (1C4-1A6), APC

            View all (31) SERPINH1 antibodies
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            Datasheet
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            Questions & Answers

            Cite SERPINH1 Monoclonal Antibody (1C4-1A6), APC

            • Antibody Testing Data (3)
            Application
            SERPINH1 Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            SERPINH1 Antibody (MA5-45301) in ICC/IF

            Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with Hsp47 monoclonal antibody (Product # MA5-45301) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 antibody. (C) Composit... View More
            View Product

            SERPINH1 Antibody in Immunocytochemistry (ICC/IF)
            SERPINH1 Antibody in Immunocytochemistry (ICC/IF)
            SERPINH1 Antibody in Western Blot (WB)

            Product Details

            MA5-45301

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            -

            Immunocytochemistry (ICC/IF)

            1:100
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Mouse / IgG1, kappa

            Class

            Monoclonal

            Type

            Antibody

            Clone

            1C4-1A6

            Immunogen

            Human HSP47, full length
            View immunogen

            Conjugate

            APC
            • Unconjugated
            • FITC
            • PE 
            • PerCP 
            • Request custom conjugation

            Excitation/Emission Max

            651/660 nm View spectra spectra

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Protein G

            Storage buffer

            95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

            Contains

            no preservative

            Storage conditions

            4°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2931755

            Product Specific Information

            1 µg/mL of MA5-45301 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects 47kDa.

            Target Information

            SERPINH1 is a member of the serpin superfamily of serine proteinase inhibitors, and its expression is induced by heat shock. The SERPINH1 protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to SERPINH1 have been found in patients with rheumatoid arthritis.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP 3; AsTP3; CBP 1; CBP 2; Cell proliferation-inducing gene 14 protein; collagen binding protein 1; Collagen-binding protein; Colligen; Colligin; Colligin 1; Colligin 2; colligin-1; colligin-2; HGNC 1547; Hsp 47; PIG 14; RA A47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)

            View more View less

            Gene Aliases: AsTP3; CBP1; CBP2; gp46; HSP47; OI10; PIG14; PPROM; RA-A47; SERPINH1; SERPINH2

            View more View less

            UniProt ID: (Human) P50454

            View more View less

            Entrez Gene ID: (Human) 871

            View more View less

            Function(s)

            serine-type endopeptidase inhibitor activity collagen binding poly(A) RNA binding unfolded protein binding protease inhibitor

            Process(es)

            chondrocyte development involved in endochondral bone morphogenesis response to unfolded protein negative regulation of endopeptidase activity collagen fibril organization collagen biosynthetic process protein maturation
            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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