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Application
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SF3b145/SAP145 Antibody (A301-606A) in IHC (P)
Detection of human SF3b145/SAP145 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-SF3b145/SAP145 (Product # A301-606A) (Lot 1) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.
Product Details
A301-606A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 825 and 872 of Human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pre-mRNA splicing factor SF3b 145 kDa subunit; Pre-mRNA-splicing factor SF3b 145 kDa subunit; SAP 145; SF3b145; spliceosome associated protein 145; Spliceosome-associated protein 145; Splicing factor 3B subunit 2; splicing factor 3b, subunit 2, 145kD; splicing factor 3b, subunit 2, 145kDa
Gene Aliases: Cus1; SAP145; SF3b1; SF3B145; SF3b150; SF3B2
UniProt ID: (Human) Q13435
Entrez Gene ID: (Human) 10992
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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