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SHANK3 Antibody (A304-178A) in WB
Detection of human and mouse SHANK3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody A304-178A (lot A304-178A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A304-178A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1475 to 1525 of Human SH3 and Multiple Ankyrin Repeat Domains 3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
SHANK3 is a major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. It interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. SHANK3 plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, SHANK3 participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. It also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. SHANK3 may be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. (Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3 variant c-3; SH3 and multiple ankyrin repeat domains protein 3 variant c-4; SH3 and multiple ankyrin repeat domains-like protein 3 variant 1; SH3 and multiple ankyrin repeat domains-like protein 3 variant 2; SH3 and multiple ankyrin repeat domains-like protein 3 variant 3; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-3; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-2; shank postsynaptic density protein; Shank3; shank3 splice variant II; shank3 splice variant III; shank3 splice variant IV; shank3e splice variant III; shank3e splice variant IV; SPANK 2; SPANK-2; SPANK2
Gene Aliases: AI841104; DEL22q13.3; Kiaa1650; PROSAP2; PSAP2; SCZD15; Shank3; Shank3b; SHANK3c-3; SHANK3c-4; SPANK-2
UniProt ID: (Mouse) Q4ACU6
Entrez Gene ID: (Human) 85358, (Mouse) 58234
MAPK cascade
embryonic epithelial tube formation
synapse assembly
learning
memory
striatal medium spiny neuron differentiation
adult behavior
negative regulation of actin filament bundle assembly
social behavior
locomotory exploration behavior
vocal learning
negative regulation of cell volume
positive regulation of long-term neuronal synaptic plasticity
brain morphogenesis
neuromuscular process controlling balance
protein oligomerization
positive regulation of synapse structural plasticity
positive regulation of synaptic transmission, glutamatergic
dendritic spine morphogenesis
positive regulation of dendritic spine development
regulation of dendritic spine morphogenesis
vocalization behavior
postsynaptic density assembly
AMPA glutamate receptor clustering
NMDA glutamate receptor clustering
guanylate kinase-associated protein clustering
regulation of long-term synaptic potentiation
positive regulation of long-term synaptic potentiation
positive regulation of glutamate receptor signaling pathway
regulation of long term synaptic depression
positive regulation of excitatory postsynaptic potential
regulation of grooming behavior
regulation of behavioral fear response
positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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