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            • Primary Antibodies ›
            • SIRT3 Antibodies

            Proteintech

            SIRT3 Polyclonal Antibody

            102 References
            View all (30) SIRT3 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite SIRT3 Polyclonal Antibody

            • Antibody Testing Data (5)
            Application
            SIRT3 Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 5

            SIRT3 Antibody (10099-1-AP) in WB

            Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10099-1-AP) SIRT3 antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.
            View Product

            SIRT3 Antibody in Western Blot (WB)
            SIRT3 Antibody in Western Blot (WB)
            SIRT3 Antibody in Western Blot (WB)
            SIRT3 Antibody in Western Blot (WB)
            SIRT3 Antibody in Western Blot (WB)

            Product Details

            10099-1-AP

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:2,000
            View 82 publications 82 publications

            Immunohistochemistry (IHC)

            -
            View 5 publications 5 publications

            Immunocytochemistry (ICC/IF)

            -
            View 11 publications 11 publications

            Immunoprecipitation (IP)

            -
            View 4 publications 4 publications
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Published species

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            SIRT3 Fusion Protein Ag0148 (202-399 aa encoded by BC001042)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.17 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            Immunogen sequence: GNYKPNVTH YFLRLLHDKG LLLRLYTQNI DGLERVSGIP ASKLVEAHGT FASATCTVCQ RPFPGEDIRA DVMADRVPRC PVCTGVVKPD IVFFGEPLPQ RFLLHVVDFP MADLLLILGT SLEVEPFASL TEAVRSSVPR LLINRDLVGP LAWHPRSRDV AQLGDVVHGV ESLVELLGWT EEMRDLVQRE TGKLDGPDK (202-399 aa encoded by BC001042)

            Target Information

            SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; mitochondrial protein lysine deacetylase; mSIR2L3; NAD-dependent deacetylase sirtuin-3; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3

            View more View less

            Gene Aliases: 2310003L23Rik; AI848213; SIR2L3; SIRT3

            View more View less

            UniProt ID: (Human) Q9NTG7, (Mouse) Q8R104

            View more View less

            Entrez Gene ID: (Human) 23410, (Mouse) 64384, (Rat) 293615

            View more View less

            Function(s)

            NAD+ ADP-ribosyltransferase activity protein binding zinc ion binding NAD-dependent histone deacetylase activity (H3-K14 specific) NAD+ binding histone deacetylase activity protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity enzyme binding 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity metal ion binding N-acetylgalactosamine-6-phosphate deacetylase activity NAD binding diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity

            Process(es)

            protein ADP-ribosylation protein deacetylation mitochondrion organization aerobic respiration peptidyl-lysine deacetylation histone H3 deacetylation negative regulation of peptidyl-lysine acetylation positive regulation of insulin secretion negative regulation of ERK1 and ERK2 cascade negative regulation of reactive oxygen species metabolic process aging
            It has to be done as per old AB suggested Products section.
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