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Invitrogen
SLC7A5 Polyclonal Antibody
FIGURE: 1 / 7
SLC7A5 Antibody (PA5-115916) in ICC/IF
Immunocytochemistry analysis of SLC7A5 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115916) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115916
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLC7A5(Accession Q01650), corresponding to amino acid residues F399-V449.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900550
Product Specific Information
Antibody detects endogenous levels of total SLC7A5.
Target Information
Solute carrier family 7 member 5 also known as large neutral amino acids transporter small subunit 1 is a protein that in humans is encoded by the SLC7A5 gene.It is sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc.It involved in cellular amino acid uptake and the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.Acts as an amino acid exchanger and plays a role in neuronal cell proliferation (neurogenesis) in brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 4F2; 4F2 LC; 4F2 light chain; 4F2HC; 4T2HC; CD98 light chain; CD98HC; E16; hLAT1; Integral membrane protein E16; L-type amino acid transporter 1; large neutral amino acids transporter 1; Large neutral amino acids transporter small subunit 1; LAT1; MDU1; MP hLAT1; NACAE; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (amino acid transporter light chain, L system), member 5; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5; Solute carrier family 7 member 5; y+ system cationic amino acid transporter
Gene Aliases: 4F2LC; CD98; CD98LC; D0H16S474E; D16S469E; E16; hLAT1; LAT1; MPE16; SLC7A5; TA1
UniProt ID: (Human) Q01650, (Mouse) Q9Z127
Entrez Gene ID: (Human) 8140, (Mouse) 20539
amino acid transmembrane transporter activity
neutral amino acid transmembrane transporter activity
L-amino acid transmembrane transporter activity
antiporter activity
peptide antigen binding
L-leucine transmembrane transporter activity
L-phenylalanine transmembrane transporter activity
L-tryptophan transmembrane transporter activity
transporter
cellular amino acid metabolic process
transport
amino acid transport
nervous system development
neutral amino acid transport
L-amino acid transport
cell differentiation
leukocyte migration
L-alpha-amino acid transmembrane transport
amino acid transmembrane transport
multicellular organism development
leucine transport
phenylalanine transport
tryptophan transport
cation transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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