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SLP76 Antibody (PA5-96165) in WB
Western blot analysis of SLP76 in various lysates. Samples were incubated with SLP76 Polyclonal antibody (Product # PA5-96165) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96165
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 159-533 of human LCP2 (NP_0055561)
Conjugate
Form
Liquid
Concentration
0.64 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807967
Product Specific Information
Positive Samples: U-937, BT-474, Mouse spleen, Mouse liver; Cellular Location: Cytoplasm
Immunogen sequence: SILPAKPFPN SNSMYIDRPP SGKTPQQPPV PPQRPMAALP PPPAGRNHSP LPPPQTNHEE PSRSRNHKTA KLPAPSIDRS TKPPLDRSLA PFDREPFTLG KKPPFSDKPS IPAGRSLGEH LPKIQKPPLP PTTERHERSS PLPGKKPPVP KHGWGPDRRE NDEDDVHQRP LPQPALLPMS SNTFPSRSTK PSPMNPLPSS HMPGAFSESN SSFPQSASLP PYFSQGPSNR PPIRAEGRNF PLPLPNKPRP PSPAEEENSL NEEWYVSYIT RPEAEAALRK INQDGTFLVR DSSKKTTTNP YVLMVLYKDK VYNIQIRYQK ESQVYLLGTG LRGKEDFLSV SDIIDYFRKM PLLLIDGKNR GSRYQCTLTH AAGYP
Target Information
SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 76 kDa tyrosine phosphoprotein; Lymphocyte cytosolic protein 2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa); SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP-76 tyrosine phosphoprotein; SLP76
Gene Aliases: AI323664; BB161688; LCP2; SLP-76; SLP76; twm
UniProt ID: (Human) Q13094, (Mouse) Q60787
Entrez Gene ID: (Human) 3937, (Mouse) 16822
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