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Proteintech

SMAD5 Monoclonal Antibody (1A6A4)

4 References

View all (35) SMAD5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

Application

SMAD5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

SMAD5 Antibody (67052-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

67052-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:3,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:300

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1A6A4

Immunogen

SMAD5 Fusion Protein Ag28699

Conjugate

Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

miR-122-5p targets GREM2 to protect against glucocorticoid-induced endothelial damage through the BMP signaling pathway.

Molecular and cellular endocrinology

Huang X,Jie S,Li W,Li H,Ni J,Liu C

Tue Mar 15 00:00:00 UTC 2022

Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway.

Applied bionics and biomechanics

Yang S,Yao L,Wang X,Sun H,Du C,Song C,Fu J,Wu Y,Huang H,Wang C,Wang Y,Xie Y

Fri Aug 25 00:00:00 UTC 2023

HIF-1α plays an essential role in BMP9-mediated osteoblast differentiation through the induction of a glycolytic enzyme, PDK1.

Journal of cellular physiology

Amir MS,Chiba N,Seong CH,Kusuyama J,Eiraku N,Ohnishi T,Nakamura N,Matsuguchi T

Fri Apr 01 00:00:00 UTC 2022

EGR1 plays an important role in BMP9-mediated osteoblast differentiation by promoting SMAD1/5 phosphorylation.

FEBS letters

Chiba N,Noguchi Y,Seong CH,Ohnishi T,Matsuguchi T

Fri Jul 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: DKFZp781C1895; DKFZp781O1323; Dwarfin-C; Dwf-C; hSmad5; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 5; mSmad5; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5

Gene Aliases: 1110051M15Rik; AI451355; Dwf-C; DWFC; JV5-1; MADH5; Msmad5; MusMLP; SMAD5

UniProt ID: (Human) Q99717, (Mouse) P97454, (Rat) Q9R1V3

Entrez Gene ID: (Human) 4090, (Mouse) 17129, (Rat) 59328

Function(s)

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding metal ion binding DNA binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

ureteric bud development Mullerian duct regression osteoblast fate commitment transcription, DNA-templated protein phosphorylation signal transduction transforming growth factor beta receptor signaling pathway germ cell development embryonic pattern specification erythrocyte differentiation BMP signaling pathway positive regulation of osteoblast differentiation positive regulation of transcription, DNA-templated cartilage development cardiac muscle contraction bone development SMAD protein signal transduction cellular response to organic cyclic compound positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus angiogenesis regulation of transcription, DNA-templated multicellular organism development cell differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to BMP stimulus ossification regulation of ossification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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