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Invitrogen

SMAD7 Polyclonal Antibody

Name: SMAD7 Polyclonal Antibody
Brand: Invitrogen
SKU: PA141506
Price: 12139.38 MXN

6 Published Figures

8 References

View all (22) SMAD7 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Published Figures (6)

Application

SMAD7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

SMAD7 Antibody (PA1-41506) in IHC (P)

Immunohistochemical analysis of SMAD7 in formalin fixed paraffin-embedded (FFPE) human liver. Samples were incubated in SMAD7 polyclonal antibody (Product # PA1-41506). Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated wit... View More

SMAD7 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-41506

Applications

Tested Dilution

Publications

Western Blot (WB)

4-6 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Sheep

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Two peptides made from within amino acids 10 and 50 of human SMAD7

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2193487

Product Specific Information

This antibody is predicted to react with chimpanzee, canine, pufferfish, xenopus and zebrafish based on sequence homology.

Suggested positive control: A549, HepG2.

Target Information

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genome-Wide Analysis of Smad7-Mediated Transcription in Mouse Embryonic Stem Cells.

International journal of molecular sciences

Meng G,Lauria A,Maldotti M,Anselmi F,Polignano IL,Rapelli S,Donna D,Oliviero S

PA1-41506 was used in Western Blotting to establish Smad7 as a new, integral component of the regulatory circuitry that controls ESC identity.

Sat Dec 18 00:00:00 UTC 2021

miR-21 mediates nickel nanoparticle-induced pulmonary injury and fibrosis.

Nanotoxicology

Mo Y,Zhang Y,Wan R,Jiang M,Xu Y,Zhang Q

PA1-41506 was used in Western Blot to study exposure of mice to nickel particles in lung inflammation and fibrosis.

Sun Nov 01 00:00:00 UTC 2020

Matrine suppresses cardiac fibrosis by inhibiting the TGF‑β/Smad pathway in experimental diabetic cardiomyopathy.

Molecular medicine reports

Zhang Y,Cui L,Guan G,Wang J,Qiu C,Yang T,Guo Y,Liu Z

Published figure using SMAD7 polyclonal antibody (Product # PA1-41506) in Western Blot

Mon Jan 01 00:00:00 UTC 2018

Protective effect of curcumin against ultraviolet A irradiation‑induced photoaging in human dermal fibroblasts.

Molecular medicine reports

Liu X,Zhang R,Shi H,Li X,Li Y,Taha A,Xu C

Published figure using SMAD7 polyclonal antibody (Product # PA1-41506) in Western Blot

Tue May 01 00:00:00 UTC 2018

Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage.

PloS one

Garrison P,Yue S,Hanson J,Baron J,Lui JC

Published figure using SMAD7 polyclonal antibody (Product # PA1-41506) in Immunohistochemistry

Wed Sep 13 00:00:00 UTC 2017

NIM811 downregulates transforming growth factor‑β signal transduction in vivo and in vitro.

Molecular medicine reports

Chen J,Liu DG,Wang H,Wu XN,Cong M,You H,Jia JD

PA1-41506 was used in Western Blot to suggest that the antifibrotic effect of NIM811 was due to the inhibition of TGF‚β1 and its downstream signaling molecules.

Fri Jan 01 00:00:00 UTC 2016

Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.

Obesity (Silver Spring, Md.)

Beaudoin MS,Snook LA,Arkell AM,Stefanson A,Wan Z,Simpson JA,Holloway GP,Wright DC

PA1-41506 was used in western blot to study the effect of rosiglitazone treatment on the transforming growth factor-beta/SMAD signaling pathway in white adipose tissue of diabetic rats

Tue Jul 01 00:00:00 UTC 2014

Smad7 regulates terminal maturation of chondrocytes in the growth plate.

Developmental biology

Estrada KD,Wang W,Retting KN,Chien CT,Elkhoury FF,Heuchel R,Lyons KM

PA1-41506 was used in immunohistochemistry to study the role of Smad7 in modulating chondrocyte terminal maturation at the growth plate using knock out mice

Tue Oct 15 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: FLJ16482; hSMAD7; MAD (mothers against decapentaplegic, Drosophila) homolog 7; MAD homolog 7; MAD homolog 8; madh8; Mothers against decapentaplegic homolog 7; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; mothers against DPP homolog 7; mothers against DPP homolog 8; SMAD; SMAD 7; SMAD family member 7; SMAD, mothers against DPP homolog 7

Gene Aliases: CRCS3; MADH7; MADH8; SMAD7

UniProt ID: (Human) O15105, (Mouse) O35253

Entrez Gene ID: (Sheep) 101111414, (Human) 4092, (Mouse) 17131

Function(s)

transcription factor activity, sequence-specific DNA binding protein binding collagen binding beta-catenin binding transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity ubiquitin protein ligase binding type I transforming growth factor beta receptor binding transcription regulatory region DNA binding metal ion binding activin binding I-SMAD binding DNA binding DNA-binding transcription factor gene-specific transcriptional regulator

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD7 Polyclonal Antibody

SMAD7 Antibody (PA1-41506) in IHC (P)

Product Details

PA1-41506

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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