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Application
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SMARCAL1 Antibody (A301-616A) in WB
Detection of human SMARCAL1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SMARCAL1 antibody (Product # A301-616A) used for WB at 0.04 µg/mL (A) and 0.1 µg/mL (B) and used for IP at 3 µg/mg lysate. SMARCAL1 was also immunoprecipitated by rabbit anti-SMARCAL1 antibody BL4539, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Product Details
A301-616A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 904 and 954 of Human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a-like 1.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
PRKCQ is a calcium-independent, novel isoform that is important in transducing signals in T lymphocytes. Interaction of a T lymphocyte with an antigen-presenting cell results in clustering of the T cell antigen receptor (TCR) and the recruitment of a large signaling complex to the site of cell-cell contact. PRKCQ is essential for TCR-mediated activation. PKC theta plays a role in pathways that link the TCR signaling complex to the activation of NF-kappa B in mature T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-driven annealing helicase; HepA-related protein; hHARP; SMARCA-like protein 1; Sucrose nonfermenting protein 2-like 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
Gene Aliases: HARP; HHARP; SMARCAL1
UniProt ID: (Human) Q9NZC9
Entrez Gene ID: (Human) 50485
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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