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Invitrogen
SMCR8 Recombinant Rabbit Monoclonal Antibody (HL1007)
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Application
FIGURE: 1 / 4
SMCR8 Antibody (MA5-38273) in ICC/IF
SMCR8 Recombinant Rabbit Monoclonal Antibody (HL1007) detects SMCR8 protein at nucleus by immunofluorescent analysis. Sample: DIV10 rat E19 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMCR8 stained by SMCR8 Recombinant Rabbit Monoclonal Antibody (HL1007) (Product # MA5-38273) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Product Details
MA5-38273
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL1007
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SMCR8. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898189
Product Specific Information
Centrifuge briefly prior to opening.
Target Information
Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity. Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ34716; Guanine nucleotide exchange protein SMCR8; Smith-Magenis syndrome chromosomal region candidate gene 8 protein; Smith-Magenis syndrome chromosomal region candidate gene 8 protein homolog; Smith-Magenis syndrome chromosome region, candidate 8 homolog
Gene Aliases: 2310076G09Rik; AI642055; D030073L15Rik; RGD1564621; SMCR8
UniProt ID: (Human) Q8TEV9, (Mouse) Q3UMB5
Entrez Gene ID: (Human) 140775, (Mouse) 237782, (Rat) 497918
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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