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Invitrogen
SMYD3 Recombinant Superclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
SMYD3 Antibody (711549) in WB
Knockdown of SMYD3 was achieved by transfecting HeLa cells with SMYD3 specific siRNA (Silencer® select Product # s34865 and Product # s34863). Western blot analysis (Fig a) was performed using Nuclear enriched lysates from the SMYD3 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-SMYD3 Recombinant Rabbit Polyclonal Antibody (Product # 711549, 1-2 µg/mL) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (P... View More
Product Details
711549
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human SMYD3 (aa1-aa428)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2692256
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Mouse
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bA74P14.1 (novel protein); Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1
Gene Aliases: bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1
UniProt ID: (Human) Q9H7B4
Entrez Gene ID: (Human) 64754
nucleosome assembly
negative regulation of protein kinase activity
myotube cell development
positive regulation of peptidyl-serine phosphorylation
histone lysine methylation
establishment of protein localization
positive regulation of transcription from RNA polymerase II promoter
cellular response to dexamethasone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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