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Application
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SN38 Antibody (MA5-50767) in WB
Western blot analysis of SN38 with Lane 1: SN38-BSA (50 ng/lane), Lane 2: BSA (negative) (50 ng/lane), Lane 3: 293T cell lysate (negative) (20 µg/lane). Samples were incubated with recombinant monoclonal SN38 (Product # MA5-50767) at a dilution of 1:1,000 (in 5% NFDM/TBST, 4°C overnight) on 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST (1 hr, room temp), followed by Goat Anti-Rabbit IgG HRP at 1:50,000 (1 hr, room temp).
Product Details
MA5-50767
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-05
Immunogen
SN38-OVA
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3090453
Product Specific Information
Product MA5-50767 was used in a Competitive ELISA and Indirect ELISA.
Target Information
SN38 is a chemical compound that belongs to the class of topoisomerase I inhibitors. It is the active metabolite of irinotecan, a chemotherapeutic drug used in the treatment of various types of cancer, particularly colorectal cancer. SN38 exerts its anti-cancer effects by inhibiting the action of topoisomerase I, an enzyme involved in DNA replication and repair. By inhibiting this enzyme, SN38 prevents DNA from unwinding, leading to the accumulation of DNA damage and ultimately inducing cell death in cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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