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Invitrogen
SNAP91 Polyclonal Antibody
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Application
FIGURE: 1 / 1
SNAP91 Antibody (PA5-93191) in WB
Western blot analysis of SNAP91 in extracts of various cell lines. Samples were incubated with SNAP91 Polyclonal antibody (Product # PA5-93191) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-93191
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 321-600 of human SNAP91 (NP_0012297231)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.24 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806663
Product Specific Information
Immunogen sequence: AVPVSTSKPS SDLLDLQPDF SSGGAAAAAA PAPPPPAGGA TAWGGFGGSF MAPSPSPVTP AQNNLLQPNF EAAFGTTPST SSSSSFDPSV FDGLGDLLMP TMAPAGQPAP VSMVPPSPAM AASKALGSDL DSSLASLVGN LGISGTTTKK GDLQWNAGEK KLTGGANWQP KVAPATWSAG VPPSAPLQGA VPPTSSVPPV AGAPSVGQPG AGFGMPPAGT GMPMMPQQPV MFAQPMMRPP FGAAAVPGTQ LSPSPTPASQ SPKKPPAKDP LADLNIKDFL; Positive Samples: Mouse brain, Rat brain, Rat liver; Cellular Location: Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit
Target Information
Clathrin coat assembly protein AP180 is a protein that in humans is encoded by the SNAP91 gene. Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 91 kDa synaptosomal-associated protein; assembly protein 180 (AP180); Clathrin coat assembly protein AP180; Clathrin coat-associated protein AP180; Phosphoprotein F1-20; synaptosomal-associated protein 91 kDa; synaptosomal-associated protein, 91 kDa; synaptosomal-associated protein, 91kDa homolog
Gene Aliases: 91kDa; AP180; F1-20; mKIAA0656; Snap91
UniProt ID: (Rat) Q05140, (Mouse) Q61548
Entrez Gene ID: (Rat) 65178, (Mouse) 20616
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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