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Application
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SOD3 Antibody (PA5-77814) in WB
Western blot analysis of SOD3 in human cervical cancer cell lysates (HeLa) with 15 µg of sample. The sample was incubated with SOD3 polyclonal antibody (Product # PA5-77814) using a dilution of 1:1000 (2 hours at 4°C), and followed by Goat-Anti-Rabbit HRP (1 hour at RT) at a dilution of 1:2000 and ECL development (5 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder (MW), Lane 2: Human Cervical Cancer cell lysates (HeLa).
Product Details
PA5-77814
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide corresponding to AA 227-236 of human EC SOD
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2736644
Product Specific Information
1 µg/mL of PA5-77814 was sufficient for detection of ECSOD in 20 µg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects extracellular SOD approximately 35kDa.
Target Information
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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