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SOD3 Antibody (PA5-93329) in ICC/IF
Immunofluorescence analysis of Extracellular superoxide dismutase [Cu-Zn] was performed using 70% confluent log phase T-47D and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SOD3 Polyclonal Antibody (Product # PA5-93329) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibod... View More
Product Details
PA5-93329
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 19-160 of human SOD3 (NP_0030932)
Conjugate
Form
Liquid
Concentration
1.52 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806721
Product Specific Information
Positive Samples: 22Rv1, HL-60, Mouse kidney, Mouse lung; Cellular Location: extracellular space^M
^M
Immunogen sequence: WTGEDSAEPN SDSAEWIRDM YAKVTEIWQE VMQRRDDDGA LHAACQVQPS ATLDAAQPRV TGVVLFRQLA PRAKLDAFFA LEGFPTEPNS SSRAIHVHQF GDLSQGCEST GPHYNPLAVP HPQHPGDFGN FAVRDGSLWR YR
Target Information
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALS1; EC SOD; EC-SOD; extracellular superoxide dismutase; Extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD 3; Superoxide dimutase 3; superoxide dismutase; Superoxide dismutase B; testicular tissue protein Li 175
Gene Aliases: AI314465; EC-SOD; ECSODPT; Sod-3; SOD3
UniProt ID: (Human) P08294, (Rat) Q08420, (Mouse) O09164
Entrez Gene ID: (Human) 6649, (Rat) 25352, (Mouse) 20657
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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